FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_CUI15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_CUI15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115153877
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG119350.026.0684935
TTCTGCT3453700.025.0112194-95
CTTCTGC4683000.023.27164694-95
TCTGCTT2479400.022.73403494-95
GTCTAGG304850.021.2373431
TATACCG317950.020.1385445
TCTTCTG6178700.020.05168594-95
GTACCGT170550.019.6351596
AGTACCG240050.019.1347065
CTGCTTG1531950.019.07763194-95
ACTGTCC178800.018.5423588
TACCGTG225350.018.2109767
GGACTGC227900.018.1122536
GTCTTCT7412000.017.86297694-95
CTAGACA336550.017.85414
GTCTATG354850.017.8159281
TAGAGCG275600.017.4593165
GTATAAG524200.017.4415321
CCTAGAC322700.017.4285183
GACGGTC153300.017.3509377