FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93063439
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3443050.026.03982794-95
TCTGCTT2410600.024.38290694-95
GTCTAGG221050.023.7127321
CTTCTGC4767950.023.67345494-95
TAGACGG103350.023.4399245
TCTTCTG6272750.020.59652194-95
TATACCG243400.020.3544885
CTGCTTG1521050.020.30422694-95
GTACCGT154900.020.300336
ACTGTCC153850.019.1413468
GTCTATG262100.019.0188731
CCTAGAC238950.018.5275383
GTCTTCT7632300.018.25384594-95
GTATAAG331700.018.2403071
AGTACCG218200.018.1337135
CTAGACA261400.017.735394
GTGTATG295200.017.7080961
ACTGAGC353300.017.4640038
CACTGAG355900.017.2296527
TACACCG193150.017.1901235
Produced by FastQC (version 0.11.5)