FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94797826
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3189600.024.28624794-95
CTTCTGC4375500.022.61444394-95
TCTGCTT2310750.022.40249494-95
TCTTCTG5694100.019.99435694-95
TAGACGG116600.019.1875155
TATACCG234450.018.781315
CTGCTTG1522000.018.61409894-95
GTCTAGG234000.018.1121981
ACTGTCC159000.017.8940328
GTCTTCT6877350.017.78783294-95
GTACCGT159150.017.7585166
AGTACCG225250.016.6171975
GTCTATG270200.015.5095741
CGTCTTC8405950.015.49031694-95
CCTAGAC239350.015.3409423
TGCTTGA1046500.015.30718494-95
GTATAAG341100.014.9214631
TACCGTG212350.014.8746877
GTGTATG299300.014.8598421
TAGAGCG244750.014.65285
Produced by FastQC (version 0.11.5)