FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69285670
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1896200.015.51307694-95
GCCGTCT2398050.015.47832594-95
TGCCGTC2931450.014.18501794-95
ATGCCGT3106450.012.87903894-95
CGTCTTC1850350.012.42303394-95
TATACCG194250.012.2510395
TTCTGCT948650.012.22201394-95
GTCTTCT1415800.012.14304794-95
GTCTAGG187750.012.0345611
TATGCCG3463250.011.97455692-93
TAGAGCG232550.011.8265595
TATACTG252800.011.7999125
TAGACGG116150.011.6143615
TCTGCTT880150.011.55962794-95
TGCTTGA558250.011.53325394-95
CCTAGAC192250.011.4150913
ACTGTCC194600.011.0813168
GCTTGAA441950.011.01619894-95
GTATGCC3668550.010.9283192-93
CGTATGC3811750.010.8616490-91
Produced by FastQC (version 0.11.5)