FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64693991
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT1413200.018.93963894-95
CTTCTGC1800950.017.63908294-95
TCTGCTT1129450.017.10784594-95
TCTTCTG2230050.016.42607394-95
GTCTTCT2647400.016.1734894-95
CCGTCTT3774900.015.58186194-95
CGTCTTC3342800.015.15913294-95
GCCGTCT4536400.014.60852994-95
CTGCTTG837350.014.464869594-95
TATACCG171000.014.3056175
TGCTTGA625250.013.93623294-95
GTCTAGG173050.013.4422081
TGCCGTC5378900.013.3990494-95
GCTTGAA493800.012.87023394-95
TATACTG247850.012.72549255
GTATAAG214900.012.3296891
CCTAGAC172600.012.2194053
TAGAGCG172150.012.1958115
AGTACCG177300.012.0023075
GTACCGT142400.011.9417646
Produced by FastQC (version 0.11.5)