FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109206034
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2112100.020.76391894-95
CTTCTGC2837300.020.14250694-95
GTCTAGG270600.019.7766761
TATACCG274700.019.1419225
TCTTCTG3624950.018.7245494-95
TAGACGG150050.018.0441075
GTCTTCT4366900.017.83332694-95
TCTGCTT1633350.017.71728994-95
GTCTATG315700.017.4185431
TAGAGCG280300.016.5903745
GTACCGT212550.016.5597446
CCGTCTT6399850.016.44937994-95
CACTGAG427100.016.4370147
CGTCTTC5541000.016.26148294-95
ACTGAGC435200.016.251148
TATACTG382700.016.2223475
ACTGTCC209900.016.1797458
GTGTATG391700.015.9212691
CCTAGAC290150.015.863813
GTATAAG372300.015.8053891
Produced by FastQC (version 0.11.5)