FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91320822
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT1651200.021.92990394-95
CTTCTGC2270500.021.66887594-95
TATACCG240200.020.5663195
TAGACGG119600.020.4140015
GTCTAGG235550.020.2541351
TCTTCTG2972750.019.93979694-95
GTACCGT165250.018.655186
GTCTTCT3627800.018.6025194-95
TCTGCTT1256850.018.09664294-95
GTCTATG270350.018.0868361
AGTACCG225200.017.9286165
GTATAAG348350.017.8192981
CCTAGAC235750.017.4086533
TACACCG220100.017.3297275
ACTGAGC351150.017.165158
GTGTATG327500.017.0801581
CACTGAG348050.017.0041547
CGTCTTC4559350.016.86809794-95
TATACTG339950.016.8371355
ACTGTCC166750.016.8060178
Produced by FastQC (version 0.11.5)