FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116340858
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2196050.025.35801394-95
CTTCTGC3463100.023.98799994-95
TCTGCTT1831600.023.43166794-95
TCTGCGT534300.022.56254494-95
TTCTGCG549500.021.30309794-95
TCTTCTG4377000.021.2572194-95
GTCTGCT788650.020.89906394-95
GTCTAGG249250.020.5919421
TATACCG228700.020.1672275
GTCTTCT5359350.019.73207394-95
GTACCGT162750.019.4377656
ACTGTCC164750.019.3164378
CGTCTGC1308200.018.19415594-95
TAGACGG122200.017.9193745
CTAGACA270200.017.9135634
TACACCG216700.017.5576635
CCTAGAC269200.017.4159053
AGTACCG221150.017.3761965
CTGCTTG1035100.017.375594-95
TACACAG208500.017.3596945
Produced by FastQC (version 0.11.5)