FastQCFastQC Report
Fri 2 Oct 2020
HMYN5DRXX_n01_AZU15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYN5DRXX_n01_AZU15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70838246
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG372750.013.8462381
GTATAGG187550.012.3011121
TATACTG157400.012.1919965
TTAGGAC224400.012.0658733
GTCTATG203650.011.8191381
TATTGGG422000.011.7790772
GGACCGT315300.011.4495336
TAGGACC231500.011.2852164
TCCTATA243150.011.1117242
GTGTAGG276550.010.9396441
GTCTAGG187000.010.8110291
CTATACT201100.010.7236344
ACCGTTA165850.010.7110928
TATCTCG870150.010.69630294-95
CCTATAC192900.010.6625833
TATAGTG208250.010.5379265
TAGGACA212950.010.5060984
ACTGAGC406350.010.4499668
CTTAGGA287000.010.4250032
GTGTATG235050.010.4021371
Produced by FastQC (version 0.11.5)