FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp89.352000000b7273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp89.352000000b7273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14289001
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1448471.0136957790121228Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA191150.055.84016446
ATCTCGG201950.054.96557235
GCCGTAT197600.053.9479347
TCTCGGT204450.053.91727436
GGTCGCC200800.053.76642243
CTCGGTG208350.052.91024437
GATCTCG214400.051.7748634
TGGTCGC213000.050.62147542
TCGCCGT210100.050.40318745
CCGTATC214200.049.94681548
GAGTGTA221100.049.8926927
AGTGTAG223800.049.3220428
CGTATCA221450.048.4222449
TAGATCT232200.047.77609632
GTGGTCG228350.047.73970441
GTAGATC231750.047.6732231
AGAGTGT236100.046.73574426
GTCGCCG230450.046.71208644
GTGTAGA239500.046.24761629
AGATCTC243500.045.6755433
Produced by FastQC (version 0.11.2)