FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp88.352000000b7230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp88.352000000b7230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22734518
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7098233.1222258593738386Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT832250.062.0807847
CGCCGTA834650.061.88007746
CCGTATC848700.060.9183348
CGTATCA849100.060.8817949
GGTCGCC856150.060.59403643
TCTCGGT872750.059.8574936
CTCGGTG873800.059.79554737
TGGTCGC878300.059.14637842
ATCTCGG884200.059.1179835
GTATCAT874800.059.09085550
TCGCCGT870550.059.06266445
GTGGTCG883250.058.80698441
GATCTCG908600.057.58571234
ATCATTA911450.057.283152
TCATTAA920950.056.756353
GTCGCCG910700.056.73408544
CATTAAA925050.056.54599854
TATCATT921850.056.31154351
TAGATCT945200.055.36292332
ATTAAAA948150.055.1545655