FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp87.352000000b7209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp87.352000000b7209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12654969
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG144617211.427700850156173Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT204960.1619600964648748Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGTAGATCTCGGTGGTCGCCGT150440.11887820507501835Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1641100.065.3760847
ATCTCGG1664300.065.21503435
CGCCGTA1645400.065.17152446
TCTCGGT1664750.065.1561836
CCGTATC1647250.065.1527348
CTCGGTG1666800.065.0615637
CGTATCA1654250.064.9769949
GGTCGCC1660550.064.9007343
GATCTCG1674850.064.8747334
GAGTGTA1665450.064.8360627
TAGATCT1678400.064.7461132
AGTGTAG1668100.064.7435528
GTATCAT1662600.064.6967750
AGAGTGT1673950.064.4674226
GTGGTCG1674500.064.4151541
TGGTCGC1673850.064.3978442
AGATCTC1687750.064.3604333
GTAGATC1684050.064.3382131
GTGTAGA1684650.064.3147929
TGTAGAT1688850.064.21751430
Produced by FastQC (version 0.11.2)