FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp86.352000000b71d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp86.352000000b71d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10622742
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7352926.921866312859712Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT306760.28877666425486Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA866550.062.19985627
ATCTCGG872050.062.17209235
GCCGTAT860850.062.02192347
TCTCGGT875450.061.8746736
AGTGTAG871850.061.8137128
TAGATCT878650.061.7347132
CTCGGTG877750.061.6900737
CCGTATC866350.061.6685748
AGAGTGT873800.061.63768826
GATCTCG880900.061.59262534
CGTATCA870700.061.4457549
CGCCGTA869850.061.35491646
TAGGGAA897600.061.2852119
GTGTAGA882400.061.28227629
GTATCAT874200.061.25406350
GTAGGGA902000.061.2104218
GTAGATC885250.061.14794531
GGTCGCC877350.061.12300543
TGTAGAT887700.060.98706430
TGTAGGG904450.060.97496817
Produced by FastQC (version 0.11.2)