FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp85.352000000b71ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp85.352000000b71ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15934632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2834191.7786353647828201Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA334800.061.95362546
ATCTCGG342700.061.11525335
GCCGTAT340050.061.00933547
TCTCGGT348000.060.18409336
GGTCGCC347050.059.93673743
CTCGGTG349900.059.8197237
GATCTCG355850.058.97537634
AGTGTAG357150.058.47208428
GAGTGTA357800.058.40498427
TCGCCGT354700.058.19130745
CCGTATC357450.058.09807648
TGGTCGC358900.057.97800442
GTGGTCG370550.056.20258741
TAGATCT375350.055.96868532
AGAGTGT374000.055.826626
CGTATCA372700.055.8156249
GTAGATC375750.055.77062631
GTCGCCG377000.055.0173844
GTGTAGA383700.054.5977129
AGATCTC391200.053.8006233
Produced by FastQC (version 0.11.2)