FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp84.352000000b7174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp84.352000000b7174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17277323
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9063855.2460962847079955Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG1086300.064.7282935
CGCCGTA1071250.064.51446
TCTCGGT1089450.064.469936
GCCGTAT1071100.064.4324947
CTCGGTG1095950.064.1228137
GAGTGTA1092750.064.1119827
GGTCGCC1086250.063.9440143
GATCTCG1101700.063.8701734
AGTGTAG1097050.063.8700728
CCGTATC1081400.063.82165548
TAGATCT1110600.063.39893332
CGTATCA1094800.063.21685849
AGAGTGT1107500.063.1904726
GTAGATC1112500.063.1627831
GTGGTCG1104650.063.0799541
GTGTAGA1115300.062.98224329
TGGTCGC1105800.062.86840442
TCGCCGT1094900.062.62789545
TGTAGAT1124350.062.54377430
GTATCAT1109100.062.43973550
Produced by FastQC (version 0.11.2)