FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_mrp82.352000000b7131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_mrp82.352000000b7131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11248423
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6216875.526881412621129Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT716300.064.5213147
CGCCGTA720450.064.118546
ATCTCGG730300.063.95754635
TCTCGGT730450.063.9207436
CTCGGTG732600.063.74440437
GGTCGCC728450.063.67843
CCGTATC729650.063.32612248
GATCTCG739050.063.3100734
CGTATCA732550.063.1524349
GTATCAT737600.062.74629250
TAGATCT747000.062.65034532
GAGTGTA743500.062.5944827
AGTGTAG744150.062.53980328
TGGTCGC743300.062.51991742
GTGGTCG745500.062.3025641
ATCATTA752850.062.04242352
TCGCCGT741350.061.9985145
GTAGATC752850.061.96856331
AGATCTC755100.061.96195633
CATTAAA755150.061.8095554
Produced by FastQC (version 0.11.2)