FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las53.352000000b73a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las53.352000000b73a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9748012
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG323440.33180098670375047Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT59350.040.98290347
CGCCGTA60650.040.5081846
GGTCGCC62600.039.35593443
TGGTCGC63750.038.5914842
GTGGTCG66250.037.1353941
CCGTATC66200.036.7950948
GTCGCCG67500.036.39529444
CGTATCA68750.035.53213549
TCGCCGT69750.034.92164645
CTCGGTG71900.034.2660937
CGGTGGT73500.033.61504439
GGTGGTC75000.033.03606440
GATCTCG76300.032.56426234
ATCTCGG76450.032.45409435
TCGGTGG78400.031.60337838
TCTCGGT80700.030.6149936
GTATCAT83700.029.39330550
GAGTGTA86550.028.18323927
AGATCTC88800.028.09898833
GTAGGGA89700.027.73788818
Produced by FastQC (version 0.11.2)