Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l06n02_las52.352000000b7372.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6103851 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 94178 | 1.5429275714626716 | Illumina Single End PCR Primer 1 (100% over 50bp) |
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 56925 | 0.9326079552072946 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTAT | 17455 | 0.0 | 40.199554 | 47 |
CGCCGTA | 17470 | 0.0 | 40.12333 | 46 |
CCGTATC | 17825 | 0.0 | 39.423695 | 48 |
GGTCGCC | 18045 | 0.0 | 39.250782 | 43 |
CGTATCA | 17940 | 0.0 | 39.210957 | 49 |
TGGTCGC | 18240 | 0.0 | 38.794376 | 42 |
TCGCCGT | 18015 | 0.0 | 38.792942 | 45 |
GTGGTCG | 18360 | 0.0 | 38.636124 | 41 |
GTCGCCG | 18410 | 0.0 | 38.416195 | 44 |
CTCGGTG | 18935 | 0.0 | 37.77798 | 37 |
CGGTGGT | 18860 | 0.0 | 37.742653 | 39 |
GGTGGTC | 18915 | 0.0 | 37.53947 | 40 |
GTATCAT | 18900 | 0.0 | 37.254494 | 50 |
ATCTCGG | 19430 | 0.0 | 36.921196 | 35 |
GATCTCG | 19560 | 0.0 | 36.71159 | 34 |
TCGGTGG | 19560 | 0.0 | 36.606647 | 38 |
TCTCGGT | 19680 | 0.0 | 36.363865 | 36 |
AGATCTC | 20255 | 0.0 | 35.523067 | 33 |
TAGATCT | 20275 | 0.0 | 35.43508 | 32 |
AGTGTAG | 20300 | 0.0 | 35.137177 | 28 |