FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las52.352000000b7372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las52.352000000b7372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103851
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG941781.5429275714626716Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT569250.9326079552072946Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT174550.040.19955447
CGCCGTA174700.040.1233346
CCGTATC178250.039.42369548
GGTCGCC180450.039.25078243
CGTATCA179400.039.21095749
TGGTCGC182400.038.79437642
TCGCCGT180150.038.79294245
GTGGTCG183600.038.63612441
GTCGCCG184100.038.41619544
CTCGGTG189350.037.7779837
CGGTGGT188600.037.74265339
GGTGGTC189150.037.5394740
GTATCAT189000.037.25449450
ATCTCGG194300.036.92119635
GATCTCG195600.036.7115934
TCGGTGG195600.036.60664738
TCTCGGT196800.036.36386536
AGATCTC202550.035.52306733
TAGATCT202750.035.4350832
AGTGTAG203000.035.13717728