FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las51.352000000b7359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las51.352000000b7359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170793
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7969178.689728358278288Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1081171.1789274929659845Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1022750.058.99917647
CGCCGTA1024350.058.9439646
GGTCGCC1037550.058.6396143
CCGTATC1030250.058.57614548
TCGCCGT1023150.058.55753745
TGGTCGC1038000.058.53905542
CGTATCA1032400.058.52598649
GTGGTCG1043900.058.27860341
GTCGCCG1041050.058.23068244
GGTGGTC1048850.058.04328540
CTCGGTG1062850.057.95200737
CGGTGGT1055450.057.88321339
GTATCAT1047550.057.74783350
GATCTCG1071050.057.67791734
ATCTCGG1072650.057.57789235
TCGGTGG1071750.057.5710538
TCTCGGT1073850.057.3828836
AGATCTC1080800.057.11644433
GAGTGTA1079950.056.8726727
TAGATCT1091850.056.6032432
Produced by FastQC (version 0.11.2)