FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las50.352000000b734c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las50.352000000b734c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9950462
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3623493.6415294083832492Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT343060.3447679112788934Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT445850.059.44436347
CGCCGTA445900.059.4280646
GGTCGCC448400.059.2843343
TGGTCGC449700.059.19885342
GTGGTCG453400.058.73119441
GTCGCCG451450.058.6202444
CCGTATC452900.058.5341948
CGTATCA454250.058.42957349
TCGCCGT451600.058.3903645
CGGTGGT462050.057.69228739
CTCGGTG463650.057.6441637
GGTGGTC462500.057.61316740
GATCTCG469250.057.0437234
ATCTCGG469850.056.93363635
TCGGTGG470800.056.9322638
GTATCAT467400.056.85136850
TCTCGGT472150.056.6639936
AGATCTC480350.055.71265833
TAGATCT488400.054.87099532
AGTGTAG492100.054.06345428