Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l06n02_las50.352000000b734c.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9950462 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 362349 | 3.6415294083832492 | Illumina Single End PCR Primer 1 (100% over 50bp) |
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 34306 | 0.3447679112788934 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTAT | 44585 | 0.0 | 59.444363 | 47 |
CGCCGTA | 44590 | 0.0 | 59.42806 | 46 |
GGTCGCC | 44840 | 0.0 | 59.28433 | 43 |
TGGTCGC | 44970 | 0.0 | 59.198853 | 42 |
GTGGTCG | 45340 | 0.0 | 58.731194 | 41 |
GTCGCCG | 45145 | 0.0 | 58.62024 | 44 |
CCGTATC | 45290 | 0.0 | 58.53419 | 48 |
CGTATCA | 45425 | 0.0 | 58.429573 | 49 |
TCGCCGT | 45160 | 0.0 | 58.39036 | 45 |
CGGTGGT | 46205 | 0.0 | 57.692287 | 39 |
CTCGGTG | 46365 | 0.0 | 57.64416 | 37 |
GGTGGTC | 46250 | 0.0 | 57.613167 | 40 |
GATCTCG | 46925 | 0.0 | 57.04372 | 34 |
ATCTCGG | 46985 | 0.0 | 56.933636 | 35 |
TCGGTGG | 47080 | 0.0 | 56.93226 | 38 |
GTATCAT | 46740 | 0.0 | 56.851368 | 50 |
TCTCGGT | 47215 | 0.0 | 56.66399 | 36 |
AGATCTC | 48035 | 0.0 | 55.712658 | 33 |
TAGATCT | 48840 | 0.0 | 54.870995 | 32 |
AGTGTAG | 49210 | 0.0 | 54.063454 | 28 |