FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las49.352000000b7308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las49.352000000b7308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29456997
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG980860.33298031024683206Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT340960.11574839078131419Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA206850.034.68331546
GCCGTAT211500.033.88865747
CATTAAA232500.031.29432154
TAGATCT248500.029.20739632
AGTGTAG250300.028.77516628
CTCGGTG255350.028.72601937
GTGTAGA252400.028.57623329
GTGGTCG261750.027.90364641
ATCATTA263600.027.74905652
TCATTAA262200.027.72317953
TGGTCGC266600.027.39597342
TCTCGGT266100.027.34093936
GGTGGTC274650.026.72028440
GTTTGAG283900.026.686281
TGTAGAT276900.026.21229730
CGTATCA278700.026.16959649
GAGTGTA275700.026.13691927
CCGTATC279050.026.01113548
CGGTGGT285100.025.82680939
ATTAAAA284200.025.81140155
Produced by FastQC (version 0.11.2)