FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las48.352000000b72d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las48.352000000b72d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21838216
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1689870.773813208917798Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA232500.052.9830746
GCCGTAT236100.052.05828547
AGTGTAG247200.049.91983428
TAGATCT249800.049.7337632
GTGTAGA254950.048.66117529
CATTAAA256250.048.59340754
TCATTAA257550.048.36182853
GTGGTCG256400.048.26303541
ATCATTA262300.047.4331152
GTATCAT260850.047.38525850
TCTCGGT262450.047.18864436
CTCGGTG265400.046.75824737
GAGTGTA265000.046.63276327
CGTATCA265250.046.49591449
GTAGATC274400.045.31390831
TGTAGAT275900.045.13096630
TGGTCGC276550.045.050242
CCGTATC278500.044.27094348
GGTGGTC282000.044.07966640
ATCTCGG283300.043.90099335
Produced by FastQC (version 0.11.2)