FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n02_las47.352000000b72aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n02_las47.352000000b72aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30313680
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2447370.8073483654904321Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA351000.052.6439146
GCCGTAT352000.052.55504647
TCATTAA377650.049.4775653
CATTAAA378850.049.3863254
GTGGTCG381600.048.73355541
ATCATTA385650.048.4426752
GTATCAT386900.047.92109350
CGTATCA391400.047.3720849
TAGATCT400550.046.66402432
CTCGGTG407850.045.8711737
CCGTATC404050.045.82810248
AGTGTAG406200.045.7065328
TGGTCGC409750.045.53927242
GTGTAGA412150.045.19811629
TCTCGGT414900.045.0648636
GGTGGTC415650.044.7660540
CGGTGGT427300.043.73426439
ATTAAAA431400.043.4199755
GAGTGTA429150.043.3030227
GGTCGCC433300.043.04754643
Produced by FastQC (version 0.11.2)