FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp89.351000000b7276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp89.351000000b7276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14289001
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1358070.9504303344929432TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACA202100.055.10873834
CGGCTAC203000.054.93242333
ACGGCTA209100.053.68178632
CATCTCG185600.053.4388139
TATGCCG213400.052.64856746
GCTACAT191800.051.808635
GTATGCC216850.051.68200745
CACGGCT217250.051.63572331
CGTATGC219000.051.18684844
CTCGTAT219750.050.93251842
TCACGGC221050.050.7466530
TCGTATG223100.050.324643
ACATCTC198400.050.11567338
CTACATC199450.049.8893836
ATGCCGT219500.049.8155147
TCTCGTA201100.049.7040441
AGTCACG227000.049.53361528
ATCTCGT205150.048.61976240
GTCACGG234850.047.7920329
TGCCGTC228900.047.4945548
Produced by FastQC (version 0.11.2)