FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp88.351000000b7233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp88.351000000b7233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22734518
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC6383632.8079020632854412TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAGCTCGTATGC284640.12520168670389228TruSeq Adapter, Index 10 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG746150.062.7676739
CTCGTAT844950.062.29263342
TCGTATG851850.062.1249643
GTATGCC851050.062.0004945
CGTATGC854650.061.9582844
TATGCCG858000.061.640946
TCTCGTA766900.061.44908541
ATCTCGT769950.061.0639540
GCCGTCT840300.060.77409749
ATGCCGT855900.060.36989247
TGCCGTC870150.059.16391448
TTATCTC796400.058.80143738
CCGTCTT866650.058.66362850
TAGCTTA890850.058.6575134
CTGCTTG899650.058.29516657
CTTATCT808950.057.8202337
GCTTATC810800.057.62163536
ACTAGCT917550.057.3965732
AGCTTAT818050.057.28840335
CACTAGC937450.056.4619331
Produced by FastQC (version 0.11.2)