FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp87.351000000b720c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp87.351000000b720c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12654969
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC12610209.964623382325156TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC816950.6455566979263244TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC675620.5338772461631475TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC533480.4215577296159319TruSeq Adapter, Index 9 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC173110.13679211699372792TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1644200.066.5169242
TCGTATG1662600.066.4438743
TCTCGTA1418750.066.3632541
GATCTCG1415500.066.2915239
GTATGCC1665250.066.1463345
CGTATGC1673650.066.0825644
AGATCTC1420650.066.08012438
ATCTCGT1421250.065.9948140
TATGCCG1673250.065.9218546
GATCAGA1671400.065.6961334
GCCGTCT1616700.065.6276349
CACGATC1683600.065.5316631
GTCACGA1681850.065.50648529
AGTCACG1683400.065.48283428
CAGATCT1433050.065.4542537
CGATCAG1687450.065.437933
ATGCCGT1647200.065.4368947
TCACGAT1686000.065.3239530
ACGATCA1691500.065.3042332
CCGTCTT1618300.065.2910850
Produced by FastQC (version 0.11.2)