FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp86.351000000b71d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp86.351000000b71d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10622742
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC6611876.224259235515651TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAGCTCGTATGC331500.3120663195999677TruSeq Adapter, Index 8 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC272080.25612972620440183TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGC267260.2515922913311836TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACCTCGTATGC216030.20336557171396988TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA766250.063.2378741
CTCGTAT864550.063.067342
TCGTATG870650.063.03545843
AATCTCG769650.062.69812839
ATCTCGT771350.062.69694540
GTCACAC874100.062.6635929
TCACACT875900.062.58259630
AGTCACA876900.062.56003628
CACTTGA878700.062.3607533
GTATGCC878050.062.3072645
ACACTTG881250.062.29150432
CGTATGC885150.062.08192444
GAATCTC779200.061.97693638
CACACTT887050.061.9157831
TATGCCG886900.061.8351946
GCCGTCT848350.061.77771449
CAGTCAC889750.061.6093327
ATGCCGT873550.061.27797747
CCGTCTT853950.061.05922350
GAACTCC905900.061.0312221
Produced by FastQC (version 0.11.2)