FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp85.351000000b71ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp85.351000000b71ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15934632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2452211.5389185015380336TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG301550.060.9298639
TATGCCG351400.060.3178246
GTATGCC352450.059.98954845
CTCGTAT352950.059.65384342
CGTATGC356150.059.56025344
TCGTATG362250.058.48967443
CAGATCA357750.058.3229134
ATGCCGT356450.058.09953347
TCTCGTA322450.057.481541
TGCCGTC360600.057.1686548
GCCGTCT364850.056.109449
ATCTCGT331200.055.88839340
CAGTCAC375000.055.79738227
ACTCCAG379550.055.43659223
GAACTCC386600.054.632521
CCGTCTT379200.053.72429350
CTGCTTG394000.053.40914557
TCATCTC347100.053.21867438
AGATCAT347350.053.206735
CTCCAGT398700.052.6248524
Produced by FastQC (version 0.11.2)