FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp84.351000000b7177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp84.351000000b7177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17277323
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8511104.926168249560421TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCGTATGC386290.22358209081349TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC325900.1886287592122923TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC280250.1622068418816966TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG961800.065.93333439
CTCGTAT1094950.065.03001442
TCGTATG1102500.064.8259443
TCTCGTA985500.064.63798541
CGTATGC1110400.064.4246244
AGTCACG1104250.064.4211728
ATCTCGT989400.064.2930940
GTATGCC1109050.064.27602445
GTCACGC1105850.064.16135429
TATGCCG1114700.064.09762646
ATATCTC991800.063.95939638
TCACGCC1107500.063.85214630
CAGTCAC1115800.063.67278727
ACGCCAA1117050.063.43232332
CACGCCA1116550.063.3698231
GCCGTCT1089800.063.2752849
ATGCCGT1104000.063.1847947
ACTCCAG1136550.062.9026623
GAACTCC1139650.062.8184321
TGCCGTC1111050.062.58800548
Produced by FastQC (version 0.11.2)