FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp83.351000000b7141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp83.351000000b7141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12041996
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2368211.9666257985802353TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC145010.12042023598081249TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT341750.060.6876142
GATCTCG299700.059.9171839
TCGTATG351500.059.56182543
CACACAG346650.059.47199231
GTATGCC352650.059.22216845
CGTATGC354950.059.02234344
TATGCCG355900.058.74996646
GTCACAC351950.058.38260329
TCTCGTA309150.058.27955241
TCACACA354750.057.9362330
CACAGTG356650.057.85354633
ATCTCGT313850.057.21627440
AGTCACA361650.057.0744328
ATGCCGT359750.056.89655347
GTGATCT314500.056.889937
ACACAGT364950.056.633732
TGATCTC317000.056.57396738
GCCGTCT359400.056.0849849
TGCCGTC363450.056.00893448
CCGTCTT362800.055.42730750