FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_mrp82.351000000b7134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_mrp82.351000000b7134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11248423
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC5430814.8280634538726TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC227910.2026150687967549TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGC190340.1692148312701256TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACCTCGTATGC156780.13937953791389246TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT719050.064.526642
TCGTATG727700.064.3078743
TCTCGTA654200.063.8719341
GTATGCC733350.063.71475245
CGTATGC736350.063.6617644
AATCTCG654600.063.56302639
TATGCCG738250.063.3819346
ATCTCGT658550.063.2570240
ATGCCGT730600.062.5796747
GCCGTCT722200.062.53185349
AGTCACT748950.062.40377828
ACTGACC749200.062.1354332
CCGTCTT726100.061.8972650
CTGCTTG748200.061.8213957
TGCCGTC737600.061.61063448
CACTGAC760050.061.51552631
CAGTCAC759150.061.26106327
GTCACTG764500.061.11593629
CTGACCA762150.060.9502633
TCACTGA768500.060.78339430
Produced by FastQC (version 0.11.2)