FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las53.351000000b73ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las53.351000000b73ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9748012
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT267000.2739020017620003TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG53400.044.6361335
CCGTCCC56300.042.70973234
GTCCCGA56650.041.82650836
CCCGTCC60550.039.71173533
ACCCGTC60400.039.57896832
TCACCCG63100.037.9957730
CACCCGT63300.037.87630531
GTCACCC65200.036.87688429
CCCGATC53950.036.13432338
AGTCACC71350.033.99032628
CCGATCT57250.033.8670339
TCGTATG72650.033.33786845
CGTATGC73200.033.03939446
CGATCTC58900.032.91829340
TATGCCG73350.032.7816448
CTCGTAT74800.032.3313444
GTATGCC75500.032.1262747
GATCTCG60750.031.91600841
ATGCCGT75600.031.20413249
CAGTCAC79650.030.9755827
Produced by FastQC (version 0.11.2)