FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las52.351000000b7375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las52.351000000b7375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103851
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC849631.3919573069526108TruSeq Adapter, Index 3 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC528910.8665185306784192TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATC162750.038.7778336
GGCATCT164250.038.37792237
ACTTAGG188850.038.20243532
CACTTAG188650.038.13160331
GCATCTC169400.037.31295838
TCGTATG194650.037.30854843
CGTATGC195600.037.18102644
CTCGTAT194450.037.1489442
TTAGGCA194250.037.01429734
TATGCCG195450.036.9782946
GTATGCC196150.036.8820145
ATGCCGT189750.036.779547
CATCTCG172300.036.7437739
GCCGTCT188700.036.46481749
TGCCGTC191550.036.36079848
TCTCGTA177150.036.05507341
TAGGCAT177000.035.8751835
ATCTCGT177650.035.835140
TCACTTA202600.035.5043130
CAGTCAC203050.035.47036727
Produced by FastQC (version 0.11.2)