FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las51.351000000b735c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las51.351000000b735c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170793
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC7240717.89540228418633TruSeq Adapter, Index 2 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC972271.0601809461842613TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC416130.45375574391440304TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAACTCGTATGC329570.35936914070571646TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTACCTCGTATGC286410.3123066892906644TruSeq Adapter, Index 2 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAACTCGTATGCC95140.10374239174300412TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC915000.058.89473738
GCCGTCT1026350.058.84060749
ATGCCGT1037500.058.82561547
TCGTATG1062450.058.8137743
TGCCGTC1035650.058.73467348
CGTATGC1065850.058.73123644
TATGCCG1063550.058.7265346
CTCGTAT1058400.058.71145642
GTATGCC1062250.058.68966745
CGATGTA1051850.058.56579234
CCGATGT1055600.058.53251633
TATCTCG920200.058.46873539
TGTATCT922200.058.29006637
GTCACCG1069750.058.2684729
CCGTCTT1030200.058.23751450
TCTCGTA929600.058.21321541
GATGTAT924350.058.19269635
ACCGATG1068100.058.1725632
ATGTATC923150.058.09581436
AGTCACC1074150.058.042628
Produced by FastQC (version 0.11.2)