Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l06n01_las50.351000000b734f.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9950462 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 315323 | 3.1689282366989593 | TruSeq Adapter, Index 1 (100% over 50bp) |
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC | 28825 | 0.28968504175987003 | TruSeq Adapter, Index 1 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC | 15760 | 0.15838460565951612 | TruSeq Adapter, Index 1 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGC | 15524 | 0.1560128564884726 | TruSeq Adapter, Index 1 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGC | 13851 | 0.1391995668140836 | TruSeq Adapter, Index 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGATCTC | 39825 | 0.0 | 58.142258 | 38 |
GATCTCG | 39885 | 0.0 | 58.07908 | 39 |
CTCGTAT | 45935 | 0.0 | 57.974075 | 42 |
CGTATGC | 46445 | 0.0 | 57.857426 | 44 |
TCGTATG | 46410 | 0.0 | 57.840733 | 43 |
CACGATC | 39870 | 0.0 | 57.825554 | 36 |
TATGCCG | 46485 | 0.0 | 57.66865 | 46 |
ATGCCGT | 45350 | 0.0 | 57.65268 | 47 |
ACGATCT | 40070 | 0.0 | 57.622253 | 37 |
TGCCGTC | 45295 | 0.0 | 57.54467 | 48 |
GCCGTCT | 44950 | 0.0 | 57.49578 | 49 |
GTATGCC | 46620 | 0.0 | 57.396545 | 45 |
CATCACG | 47160 | 0.0 | 56.732483 | 33 |
ATCACGA | 47155 | 0.0 | 56.360798 | 34 |
TCACGAT | 41220 | 0.0 | 56.29003 | 35 |
CTGCTTG | 47330 | 0.0 | 56.19621 | 57 |
CCGTCTT | 45900 | 0.0 | 56.180664 | 50 |
TCTCGTA | 41650 | 0.0 | 55.896835 | 41 |
ATCTCGT | 42150 | 0.0 | 55.050266 | 40 |
ACATCAC | 48815 | 0.0 | 54.99575 | 32 |