FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las50.351000000b734f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las50.351000000b734f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9950462
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC3153233.1689282366989593TruSeq Adapter, Index 1 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC288250.28968504175987003TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC157600.15838460565951612TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGC155240.1560128564884726TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGC138510.1391995668140836TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTC398250.058.14225838
GATCTCG398850.058.0790839
CTCGTAT459350.057.97407542
CGTATGC464450.057.85742644
TCGTATG464100.057.84073343
CACGATC398700.057.82555436
TATGCCG464850.057.6686546
ATGCCGT453500.057.6526847
ACGATCT400700.057.62225337
TGCCGTC452950.057.5446748
GCCGTCT449500.057.4957849
GTATGCC466200.057.39654545
CATCACG471600.056.73248333
ATCACGA471550.056.36079834
TCACGAT412200.056.2900335
CTGCTTG473300.056.1962157
CCGTCTT459000.056.18066450
TCTCGTA416500.055.89683541
ATCTCGT421500.055.05026640
ACATCAC488150.054.9957532