FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las49.351000000b730b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las49.351000000b730b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29456997
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT844840.2868045238963089TruSeq Adapter, Index 14 (97% over 44bp)
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG336210.1141358706727641No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG294870.10010185355961439TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG190550.037.6546848
ATGCCGT199550.035.20218749
GTATGCC204200.035.13772247
TATCTCG186250.033.56099741
CGTATCT194050.032.06633839
TCGTATG231750.031.32350545
CGTATGC232300.031.12859546
CTCGTAT236450.030.49146844
TGCCGTC234750.030.07538450
GTATCTC222200.028.3983140
GCCGTCT248100.027.93546751
GTCACAG278250.025.9986829
TCTCGTA250950.025.41040443
CCCGGTT80050.024.7613221
TCACAGT293200.024.57941430
TTCCGTA311600.022.93696636
GTTCCGT333400.022.89724735
AGTCACA319100.022.8117328
CCGTATC277550.022.81098738
CACAGTT319750.022.72531931
Produced by FastQC (version 0.11.2)