FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las48.351000000b72d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las48.351000000b72d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21838216
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1509570.6912515198127905TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG225750.055.0394148
GTATGCC236150.052.6752547
ATGCCGT235800.051.65455249
CGTATGC254050.049.11570746
TCGTATG253850.049.1132745
CTCGTAT260300.047.63720344
TGCCGTC259250.046.9328550
GTCACAG269350.046.45167529
CACAGTC270650.046.18159531
ACAGTCA275650.045.44548832
AGTCAAC275350.045.35538534
AGTCACA278200.045.12204428
GCCGTCT274700.043.91142351
TCACAGT287300.043.588630
GTCAACA296750.042.27341535
TCTCGTA265700.042.23001543
CAGTCAC298100.042.16856827
CAGTCAA303950.041.28317633
ACAATCT298150.037.53842539
CCAGTCA337950.037.25145726
Produced by FastQC (version 0.11.2)