FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l06n01_las47.351000000b72ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l06n01_las47.351000000b72ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30313680
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2185260.7208824530706928TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG335300.055.91917446
GTATGCC349750.053.50887745
ATGCCGT343800.053.1520247
CGTATGC373250.050.4565144
TCGTATG374950.050.22774543
TGCCGTC374100.048.69714748
CTCGTAT388850.048.05417642
GCCGTCT391300.046.20777549
GTCACCT414100.044.88657829
AGTCACC420050.044.5061128
GTAATCT379950.044.02285837
TGTAATC384700.043.32591636
TCTCGTA389300.043.17089541
TAATCTC399600.041.91820538
CTTGTAA447700.041.6944934
CACCTTG448000.041.53340531
TCACCTT470100.039.519830
CAGTCAC475150.039.3965927
TTGTAAT425650.039.37280735
ACCTTGT478600.039.09730532
Produced by FastQC (version 0.11.2)