FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_las69.352000000b7266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_las69.352000000b7266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23914934
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2531011.0583386933035233Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT342750.055.2751447
CGCCGTA344650.055.24237446
GGTCGCC359150.053.09876643
TGGTCGC360550.052.7872442
CGTATCA365500.051.7915349
CCGTATC369050.051.5173248
GTCGCCG373300.051.1887844
TCGCCGT372850.050.90989745
GTGGTCG394500.048.2006441
GTATCAT396000.047.54419350
CTCGGTG414200.046.19885337
CGGTGGT421550.045.2584439
GGTGGTC423450.045.03743440
ATCTCGG426600.044.98677435
GATCTCG429800.044.7519734
TCTCGGT429550.044.4827136
TCGGTGG435900.043.9710938
GTAGGGA442400.043.79448318
GAGTGTA446100.043.02560827
TAGGGAA458300.042.30338719
Produced by FastQC (version 0.11.2)