FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_las68.352000000b724d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_las68.352000000b724d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57867027
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG45554847.872331163652143Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4967150.066.8192947
CGCCGTA4986700.066.80559546
CCGTATC5012350.066.2804848
CGTATCA5004250.066.2763849
GTCGCCG5049350.066.0740544
GGTCGCC5065600.066.06847443
TCGCCGT5037400.066.0529445
TGGTCGC5072700.065.9132642
GTATCAT5060450.065.3234850
GTGGTCG5110400.065.31592641
CTCGGTG5139000.064.9683737
GATCTCG5170800.064.70180534
CGGTGGT5164800.064.69289439
ATCTCGG5169350.064.6703535
GGTGGTC5171050.064.6019340
GAGTGTA5167050.064.5770927
GTAGGGA5276650.064.48897618
TCTCGGT5177500.064.4808836
TCGGTGG5180700.064.4619238
TAGGGAA5305800.064.1233419
Produced by FastQC (version 0.11.2)