FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_las67.352000000b7216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_las67.352000000b7216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20732212
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG231772411.179337737815917Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGTAGATCTCGGTGGTCGCCGT216890.1046149827138561Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2517500.067.0134547
CGCCGTA2530450.066.9983446
CCGTATC2532800.066.6810548
CGTATCA2529100.066.6560449
TCGCCGT2542100.066.5743845
GTCGCCG2558250.066.4441244
GGTCGCC2574850.066.1956243
GTATCAT2542200.066.100350
TGGTCGC2576100.066.0857342
GTAGGGA2637700.065.5518918
GTGGTCG2596700.065.4553941
TAGGGAA2647350.065.2835719
CTCGGTG2605600.065.2626737
CGGTGGT2611650.065.14654539
GGTGGTC2611900.065.1427540
GATCTCG2621650.065.02186634
ATCTCGG2619950.065.0000735
TGTAGGG2656800.064.9924717
GTGTAGG2672750.064.9525416
CGTGTAG2671600.064.9034415
Produced by FastQC (version 0.11.2)