FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_las66.352000000b71fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_las66.352000000b71fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33697049
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1484197544.04532574944471Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1997610.592814522126255Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGTAGATCTCGGTGGTCGCCGT1421570.4218678021330592Illumina Single End PCR Primer 1 (96% over 30bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1082120.3211319780554078Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGT783970.2326524200976768Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGGTGTAGATCTCGGTGGTCGCCGT627900.18633679168760445Illumina Single End PCR Primer 1 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAGTGTAGATCTCGGTGGTCGCCGT363570.10789372090119821Illumina Single End PCR Primer 1 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16497350.068.848981
GTAGGGA16373650.068.06631518
CGTGTAG16431700.068.04156515
GTGTAGG16461700.068.01227616
TGTAGGG16376700.068.0068917
GCGTCGT16507550.067.9997811
TAGGGAA16391350.067.98311619
GAGCGTC16547350.067.965539
CGTCGTG16500050.067.9645812
AGCGTCG16545350.067.9517810
GTCGTGT16479450.067.93770613
AGAGCGT16609550.067.882398
TCGTGTA16446250.067.88206514
GGGAAAG16260000.067.7800121
AAGAGCG16715900.067.655587
AGGGAAA16323600.067.54613520
CGGAAGA16777500.067.4887164
TCGGAAG16795950.067.487733
GAAGAGC16769000.067.466486
ATCGGAA16882900.067.260762
Produced by FastQC (version 0.11.2)