FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_las58.352000000b7167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_las58.352000000b7167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27682668
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1526040.5512618942653937Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA216500.054.00621446
GCCGTAT220250.052.80324647
GGTCGCC222100.052.64900243
TGGTCGC228200.051.10465642
GTCGCCG238700.048.91581344
TCGCCGT242700.048.02252645
CCGTATC245750.047.48166748
CGTATCA248200.046.93834349
GTGGTCG259500.045.04863741
CTCGGTG277600.042.31599437
CGGTGGT278800.041.9812239
GGTGGTC283450.041.3779840
GTATCAT283250.040.8935650
GATCTCG294750.039.97207634
ATCTCGG299050.039.43752735
TCGGTGG298300.039.42623538
TCTCGGT306250.038.4370236
AGATCTC328000.035.9633133
AGTGTAG335700.035.1105928
TAGATCT339950.034.59659232
Produced by FastQC (version 0.11.2)