FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_las57.352000000b7124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_las57.352000000b7124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23953629
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1216720.507948085862063Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA175400.053.2729246
GCCGTAT175950.053.1104247
GGTCGCC179900.052.0150543
TGGTCGC185500.050.3135642
GTCGCCG194600.048.09566544
CCGTATC198900.047.12343648
CGTATCA202550.046.28825449
TCGCCGT202000.046.17681545
GTGGTCG207300.045.07348641
CTCGGTG222750.042.15373237
CGGTGGT226400.041.4261539
GTAGGGA229800.041.198918
GATCTCG232200.040.77230534
GGTGGTC231750.040.5294140
ATCTCGG235300.040.19577435
GTATCAT237500.039.62503450
GAGTGTA241150.039.2031427
TCGGTGG240700.039.1554438
TCTCGGT242150.038.8776536
AGTGTAG249850.037.83757428