Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l05n02_las57.352000000b7124.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23953629 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 121672 | 0.507948085862063 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 17540 | 0.0 | 53.27292 | 46 |
GCCGTAT | 17595 | 0.0 | 53.11042 | 47 |
GGTCGCC | 17990 | 0.0 | 52.01505 | 43 |
TGGTCGC | 18550 | 0.0 | 50.31356 | 42 |
GTCGCCG | 19460 | 0.0 | 48.095665 | 44 |
CCGTATC | 19890 | 0.0 | 47.123436 | 48 |
CGTATCA | 20255 | 0.0 | 46.288254 | 49 |
TCGCCGT | 20200 | 0.0 | 46.176815 | 45 |
GTGGTCG | 20730 | 0.0 | 45.073486 | 41 |
CTCGGTG | 22275 | 0.0 | 42.153732 | 37 |
CGGTGGT | 22640 | 0.0 | 41.42615 | 39 |
GTAGGGA | 22980 | 0.0 | 41.1989 | 18 |
GATCTCG | 23220 | 0.0 | 40.772305 | 34 |
GGTGGTC | 23175 | 0.0 | 40.52941 | 40 |
ATCTCGG | 23530 | 0.0 | 40.195774 | 35 |
GTATCAT | 23750 | 0.0 | 39.625034 | 50 |
GAGTGTA | 24115 | 0.0 | 39.20314 | 27 |
TCGGTGG | 24070 | 0.0 | 39.15544 | 38 |
TCTCGGT | 24215 | 0.0 | 38.87765 | 36 |
AGTGTAG | 24985 | 0.0 | 37.837574 | 28 |