FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_dj10.352000000b7322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_dj10.352000000b7322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35467726
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATC955355126.935899414583275No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTC755685721.306291246300933No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTT9717002.7396738093668596No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAA9646212.7197148190442206No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAG4616631.3016425129708062No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGT4580151.2913571058939612No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTG4279861.2066914016421577No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGG4224381.1910490117127892No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATT3898261.0991006302462132No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAT3785721.067370374971319No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAA3734181.0528388541176843No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTC3733481.0526414915915387No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAT3644951.0276807709634386No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATA3487410.9832629247220417No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCT3272660.9227149211652306No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGA3188510.8989891260578703No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTT3174380.8950052224943883No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAT3025870.8531333528402695No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACA2853040.8044045451349208No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCA2827030.7970711175562819No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAC2746770.7744420942013593No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCC2651890.7476910135146527No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTA2505230.7063407448224901No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTA2478180.6987140929192923No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTG2268030.6394630431057238No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAA2220820.6261523504495327No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACT2170660.6120099157188706No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAG2119720.5976475627447895No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATG2084930.5878386451953531No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCT2047170.5771923466421276No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAC2019460.569379610071421No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAC2017340.5687818835636658No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAG2009840.5666672850692487No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCG1975790.5570670079045947No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGC1972570.5561591402843249No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGA1934080.5453070208109762No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCA1903270.5366202501959105No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGT1896570.5347312088742312No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGT1872220.5278658124290234No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCC1619250.45654181494466267No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAA1510950.42600701268527896No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACC1369640.38616515758580067No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGA1257820.3546379037663706No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCA1213490.3421392169320356No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAT1175890.33153802981335767No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGC1148790.3238972805868637No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACG1135580.3201727677720303No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCG1098160.3096223310172183No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTT995920.2807961243413237No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGG992000.2796908941949084No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGG847370.23891297682856805No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAG823210.2321011502118856No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGC802130.2261577187102438No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGA798830.22522729537270023No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGT752620.21219854918243136No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTG745280.21012906212256177No Hit
NGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATC706150.19909649691102269No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTA617250.17403145609053144No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTC585900.16519243438386774No Hit
NGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTC555550.15663535914312635No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAC511520.14422125624856807No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGC466670.1315759572519535No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCT446250.12581861041782041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACAT34030000.070.411141
GTACCTC8296300.069.8778744
GAACATT34199000.069.799962
GTACCAT338650.069.768944
TACCTCC8137200.069.73768645
GTACATC10224800.069.7247744
AACATTG34131350.069.718733
ACCTCCT8088450.069.7095446
CATTGTG34123600.069.688955
GTACCCG127500.069.6716544
GTACTCA314450.069.66553544
GTACCAA417750.069.6511444
GTACAAG496550.069.6484244
ACATTGT34189300.069.6481554
ATTGTGG34015550.069.637696
GTACCCT225650.069.62263544
CCTCCTA8010200.069.621147
TTGTGGT34010950.069.617377
CTCCTAA8015350.069.5917748
GTACCCA207750.069.5784944
Produced by FastQC (version 0.11.2)