FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_dj09.352000000b72fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_dj09.352000000b72fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26917532
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG1228679845.6460792913704Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG13865495.151100033985285No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGGGTAG11610714.313437799572412Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGGAGAAAAACGTTGTCTTCGCAAGCTTAGATCGGAA4818201.78998579810363No Hit
AAAGTGGTAAGGTTGTCTTCGCCAGCTTAGATCGGAAGAGCGTCGTGTAG4459801.6568383758213794Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGGCGTGTAG3189291.184837450922321Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAAAAACGTTGTCTTCGCAAGCTTAGATCG2561510.951613988979376No Hit
AAAGTGGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1873970.6961893831871362Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAACGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAGGGAAA1720370.6391262022090287Illumina Single End PCR Primer 1 (100% over 27bp)
AAAGTGGTAAGGTTGGAGAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT1558480.5789832440804751Illumina Single End PCR Primer 1 (100% over 32bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGGAG1335220.49604101891659313Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGGAGAGCGTCGTGTAG1020270.3790354925555582Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCCAGCTTAGATCGGAAGAGCGTCGGGTAG1015310.37719282733647347Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGGGGAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT950870.3532530396917518Illumina Single End PCR Primer 1 (97% over 36bp)
AAAGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG894090.3321589809942457Illumina Single End PCR Primer 1 (100% over 30bp)
NAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG853920.3172356217501664Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGACGTGTAG753720.28001081228397906Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGGCGGGTAG700720.26032104280585605No Hit
AAAGTGGTAAGGGTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG645240.23970994071819066Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGGAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG624970.23217953265551985No Hit
AAAGTGGTAAGGTTGGGAAGAGTGTAGATCGTGGCGTAAAAACGTTGTCT531430.19742894705205516No Hit
AAAGTGGTAAGGTTGGACGGTGTGCGTATCAAAAACGTTGTCTTCGCAAG465240.17283902550947092No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGGGGAG460090.17092577432433256No Hit
AAAGTGGTAAGGTTGGAGAAGAAAAAACGTTGTCTTCGCAAGCTTAGATC428630.15923822436618634No Hit
AAAGTGGTAAACGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTA404120.15013263474526567Illumina Single End PCR Primer 1 (100% over 21bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAGAGCGTCGTGTAGG380430.14133167929362914No Hit
AAAGTGGTAAGGGTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG367430.13650211319522162No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCCGAAGAGCGTCGTGTAG366560.1361789037717128Illumina Single End PCR Primer 1 (95% over 22bp)
ACAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG364250.13532072702653425Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCCAGCTTAGATCGGAAGAGCGGCGTGTAG360540.1339424431630656Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTGAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG354170.13157595577484593Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAGTGTAAAAACGTTGTCTTCGCAAGCTTA345620.12839958730243173No Hit
AAAGTGGTAACGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG343020.12743367408275022Illumina Single End PCR Primer 1 (100% over 22bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG329980.12258924778096299Illumina Single End PCR Primer 1 (100% over 50bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGGAGAGCGTCGGGTAG316340.11752191842848No Hit
AAAGTGGTAAGGTTGGAGAAGAGTGTAGATCTCGGTGGTCGCCGTATCAA315280.11712812303891754Illumina Single End PCR Primer 1 (96% over 31bp)
AAAGTGGTAAGGGTGGAGAAAAACGTTGTCTTCGCAAGCTTAGATCGGAA306580.11389602880382943No Hit
AAAGTGGTAAGGTTGTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAGGGA305460.11347994310919739Illumina Single End PCR Primer 1 (100% over 25bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAA293930.10919648948499439Illumina Single End PCR Primer 1 (95% over 21bp)
AAAGTGGTAAAGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG282620.10499476697937984Illumina Single End PCR Primer 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGG24824400.070.356281
CGCATCT1965150.069.81258449
TCGCATC1979550.069.79211448
GCATCTC1980350.069.7914950
AAGTGGT24751750.069.747452
GTCGCAT1978050.069.7351247
AGTGGTA24480050.069.730513
ATGTCGC2013150.069.72043645
TGGTAAG24217250.069.6933755
GTGGTAA24479550.069.657214
TAAGGTT23738700.069.562228
GTAAGGT23834450.069.552187
GGTAAGG24360450.069.543386
AAGGTTG23931300.069.5080269
AGGTTGT17482050.069.4770110
ACAGTGG194500.069.355971
GGTTGGA5861300.069.33987411
TTGGAGA5667200.069.3004913
GTTGGAG5752900.069.2903312
AGGTTGG6575500.069.2760410
Produced by FastQC (version 0.11.2)