FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_dj08.352000000b72c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_dj08.352000000b72c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11806138
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT889604875.35104197494557No Hit
NTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT634890.5377626451596619No Hit
CTTGCAAACACTGGTCAATGAGCTTTCGAAACTAGGCTTTGAAGATCGGA597460.506058797550901No Hit
CTTGCAAACACTGGTCAATGAGCTTTGGTATTTGAACTTTCAAAGAAACT513790.4351888822576867No Hit
CTTGCAAACACTGGTCAATGAAGCTTTCGAAACTAGGCTTTGAAGATCGG469130.3973611014880565No Hit
CTTGCAAACACTGGTCAATGAGAGTTGGTATTTGAACTTTCAAAGAAACT403470.34174596298976007No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATGTGAACTTTCAAAGAAACT205120.1737401341573341No Hit
CTTGCAAACACTGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTA199530.16900530893337007No Hit
CTTGCAAACACTGGTCAACGAGATTTGGTATTTGAACTTTCAAAGAAACT198450.16809053053589582No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACG187560.15886651502803034No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGCACTTTCAAAGAAACT186780.15820584174096558No Hit
CTTGCAAACACTGGTCAAGGAGATTTGGTATTTGAACTTTCAAAGAAACT178370.1510824284791521No Hit
CTTGCAAACACTGGTCAATGAGATTTGGGATTTGAACTTTCAAAGAAACT161990.13720828945079247No Hit
CTTGCAAACATGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTA148580.12584979101548702No Hit
CTTGCAAACACTGGTCAAGCTTTCGAAACTAGGCTTTGAAGATCGGAAGA147740.12513829670634039No Hit
CTTGCAAACACTGGTCAATAAGATTTGGTATTTGAACTTTCAAAGAAACT146020.12368142740665913No Hit
CTTGCAAACACGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTA144590.12247019304704045No Hit
CTTGCAAACACTGGTCAATTAGATTTGGTATTTGAACTTTCAAAGAAACT142470.12067451693347986No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG142320.12054746437827511Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGCAAACACTGGTCAATGAGGTTTGGTATTTGAACTTTCAAAGAAACT138160.11702387351392979No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAAATTTCAAAGAAACT136820.11588887068743395No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTGAAAGAAACT129660.10982422871899346No Hit
CTTGCAAACACTGGTCAATGAGATGTGGTATTTGAACTTTCAAAGAAACT129320.109536242927196No Hit
CTTGCAAACACTGGTCAATGAGACTTGGTATTTGAACTTTCAAAGAAACT125990.10671567620165036No Hit
CTTGCAAACACTGGTCAATGAGTTTTGGTATTTGAACTTTCAAAGAAACT125380.10619899581048435No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATCTGAACTTTCAAAGAAACT125310.10613970461805546No Hit
CTTGCAAACACTGGTCAATGAGAATTGGTATTTGAACTTTCAAAGAAACT123130.10429320748241297No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATATGAACTTTCAAAGAAACT122330.10361559385465426No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTTAACTTTCAAAGAAACT119930.1015827529713781No Hit
CTTGCAAACACTGGCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTA118930.10073573593667971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCAA10872250.070.319961
TGGGGCC150.002234634269.9270810
CCCGGGG150.002234634269.927089
TACCGGT150.002234634269.927089
CTACGCG150.00223542369.9208516
TTCGCCC207.977727E-569.9181858
TCCGCTG207.980071E-569.914047
TGCAAAC10914650.069.832193
GCAAACA10908150.069.8197564
CAAACAC10926400.069.780095
AAACACT10890900.069.757096
TTGCAAA10918950.069.742
CACTGGT10815700.069.7059
AACACTG10856250.069.6912167
ACACTGG10841600.069.687168
ACTGGTC10807500.069.65565510
CTAATTC10369450.069.6301849
ACTAATT10393600.069.6271848
AACTAAT10412200.069.6200747
AAACTAA10455300.069.6137146
Produced by FastQC (version 0.11.2)