FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n02_dj06.352000000b729d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n02_dj06.352000000b729d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6702195
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG168974325.211785094286277Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG165569824.703817182281327No Hit
AAAGTGGGAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG1768232.6382849200896126No Hit
AAAGTGGTAAGGTTGGAGAAAAACGTTGTCTTCGCAAGCTTAGATCGGAA1214791.8125255979570871No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGGGTAG595930.8891564629199837Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGGCGTGTAG547310.8166130648242852Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAAAAACGTTGTCTTCGCAAGCTTAGATCG469540.7005764529381792No Hit
AAAGTGGTAAGGGTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG294490.43939336292065506No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGGAG265770.39654172998547493Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGGAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG250070.37311656852717656Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGGGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG216340.32278977260434827No Hit
AAAGTGGTAAGGTTGGAGAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT150310.22426981011444755Illumina Single End PCR Primer 1 (100% over 32bp)
AAAGAGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG149770.22346410392416216No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGGCG142500.2126169113253195No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG142490.21260199084031425Illumina Single End PCR Primer 1 (100% over 50bp)
AAAGTGGTAAGGTTGTCTTCGCCAGCTTAGATCGGAAGAGCGTCGTGTAG139370.20794679951866515Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGAAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG117860.17585283627229586No Hit
NAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG117550.175390301237132Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGCAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG113280.1690192541398751No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGAACGGAAGAGCGTCGTGTAG112710.16816878649457379Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAAATGTCG110600.16502056415845853No Hit
AAAGTGGTAAAGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG106560.15899268821632315No Hit
NAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG105820.157888572325932No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAAAAAAATAACGGAAAGATGTCG98540.14702645924208413No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAAAAACGGAAAGATGTCG93810.13996906983458404No Hit
AAAGTGGTAAGGATGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG90310.13474690008273407No Hit
AAAGTGGTAAGGTTGGAGCAGACGTTGTCTTCGCAAGCTTAGATCGGAAG86740.12942028693584715No Hit
AAAGTGGTAAGGTTGGAGTCAGACGTTGTCTTCGCAAGCTTAGATCGGAA83620.12476509561419803No Hit
AAAGTGGGAAGGGTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG72770.10857636938346317No Hit
AAAGTGGTAAGGTTGGCGAAGAATAAGAATAAAATAACGGAAAGATGTCG72420.10805415240827819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCT1450.070.6001349
AAAGTGG5720600.070.349531
GCCCTTA252.8633149E-669.91402432
AAGTGGT5292000.069.784372
ATGGCGC53550.069.754245
AGTGGTA5303000.069.7538763
GTGGTAA5278950.069.699474
CGCATCT2591050.069.68732549
GCATCTC2621550.069.6804450
TCGCATC2596400.069.6693748
TGGTAAG5216550.069.659145
ATGTCGC2639750.069.6563645
GTCGCAT2586850.069.65005547
TGTCGCA2604300.069.63692546
GATGTCG2582900.069.5948544
GGTAAGG5384300.069.56596
GCGCATC50050.069.5495248
TAAGGTT5219300.069.4746558
AAGGTTG5619900.069.448449
AGGTTGT2257500.069.4309410
Produced by FastQC (version 0.11.2)