FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las69.351000000b7269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las69.351000000b7269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23914934
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2356370.9853131938394645TruSeq Adapter, Index 12 (100% over 50bp)
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTG245360.1025969797784096Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG341900.054.69529346
CGTATGC346050.054.0777344
ATGCCGT344200.053.8315847
GTATGCC351800.053.12666345
TGCCGTC350850.052.66159448
TCGTATG360200.051.83007443
GCCGTCT351450.051.8246549
CTCGTAT361400.051.57920542
CCGTCTT373900.048.94205550
CTGCTTG385850.048.70777557
GTCACCT395150.047.0424129
AGTCACC398450.046.9950928
CCTTGTA396500.046.88900833
ACCTTGT406400.045.75539432
CACCTTG407550.045.60471331
GTAATCT381350.045.57406237
TGTAATC389700.044.82150736
TGCTTGA423300.044.2364158
TAATCTC394950.044.0289538
CAGTCAC429850.044.00824427
Produced by FastQC (version 0.11.2)