FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las68.351000000b7240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las68.351000000b7240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57867027
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT41131197.107880278694808TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAGCTCGTAT1088860.18816587899011988TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTAT1005950.17383820323100407TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5064200.066.3226546
TATGCCG5063600.066.2150548
GTATGCC5066800.066.17737647
ATGCCGT4983350.066.1335449
TGCCGTC5004100.065.9839850
TCGTATG5103850.065.9526745
GCCGTCT4990850.065.9139851
CTCGTAT5097300.065.86474644
CCGTCTT5028600.065.2474652
CTGCTTG5178700.064.94710559
TCTCGTA4863550.064.648543
ATGTCAG5197950.064.59946434
CAGTCAC5166200.064.5509327
ACATGTC5220250.064.3623532
CATGTCA5210050.064.347933
CCAGTCA5169500.064.3241626
AGTCACA5213550.064.178328
ATCTCGT4917750.064.1192242
TGCTTGA5236800.064.0967360
GTCACAT5228400.064.0472229
Produced by FastQC (version 0.11.2)